{"id":499,"date":"2025-05-07T18:36:43","date_gmt":"2025-05-07T21:36:43","guid":{"rendered":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/?p=499"},"modified":"2025-05-08T16:20:18","modified_gmt":"2025-05-08T19:20:18","slug":"identification-and-evaluation-of-antioxidant-and-reference-genes-for-quantitative-real-time-pcr-in-blood-of-c-latirostris","status":"publish","type":"post","link":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/2025\/05\/07\/identification-and-evaluation-of-antioxidant-and-reference-genes-for-quantitative-real-time-pcr-in-blood-of-c-latirostris\/","title":{"rendered":"Identification and evaluation of antioxidant and reference genes for quantitative real time PCR in blood of\u00a0C latirostris."},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"499\" class=\"elementor elementor-499\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4ab06ac e-flex e-con-boxed e-con e-parent\" data-id=\"4ab06ac\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-794db11 elementor-widget elementor-widget-text-editor\" data-id=\"794db11\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.23.0 - 05-08-2024 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p><strong>Abstract<\/strong><\/p><p>The quantitative real-time polymerase chain reaction (qPCR) has been one of the most promising approaches to perform rapid and accurate quantification of DNA in various biological systems. The aim of this study was to standardized the qPCR technique for the analysis of important genes involved in the main routes of antioxidant defense against reactive oxygen species (catalase: cat and superoxide dismutase: sod) and evaluate the stability of different reference genes in blood of Caiman latirostris hatchlings. The stability of the reference genes, \u03b2-actin, glyceraldehyde 3-phosphate dehydrogenase (gapdh) and ribosomal protein L8 (rpl8) was determined using the comparative \u0394Ct, NormFinder, geNorm, BestKeeper and RefFinder. Then, cat and sod genes were normalized with each reference gene and their mRNA abundances were determined through the qPCR. Stability of genes was ranked through the different methods in the following order: \u03b2-actin, rpl8 and gapdh, under normal physiological conditions. The results reveal that cat and sod genes present a similar relative mRNA abundance with \u03b2-actin and rpl8. This is the first report of the analysis of antioxidant mRNA as potential biomarkers of oxidative stress in blood for all crocodilians species. Besides, we determined the stability of different reference genes that can be used for normalization of mRNA abundance patterns in blood of C. latirostris, without the need to sacrifice the animals.<\/p><p>\u00a0<\/p><p><strong>Keywords<br \/><br \/><br \/><\/strong><\/p><ul><li id=\"kwrd0010\" class=\"keyword\">Crocodiles<\/li><li id=\"kwrd0015\" class=\"keyword\">mRNA abundance pattern<\/li><li id=\"kwrd0020\" class=\"keyword\">Reference genes<\/li><li id=\"kwrd0025\" class=\"keyword\">Antioxidant genes<\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-cb2e065 e-con-full e-flex e-con e-child\" data-id=\"cb2e065\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6527468 elementor-widget elementor-widget-button\" data-id=\"6527468\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/doi.org\/10.1016\/j.heliyon.2021.e06253\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">VER PUBLICACI\u00d3N<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Abstract The quantitative real-time polymerase chain reaction (qPCR) has been one of the most promising approaches to perform rapid and accurate quantification of DNA in various biological systems. The aim of this study was to standardized the qPCR technique for the analysis of important genes involved in the main routes of antioxidant defense against reactive [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-499","post","type-post","status-publish","format-standard","hentry","category-publicaciones-publicaciones"],"_links":{"self":[{"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/posts\/499","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/comments?post=499"}],"version-history":[{"count":7,"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/posts\/499\/revisions"}],"predecessor-version":[{"id":547,"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/posts\/499\/revisions\/547"}],"wp:attachment":[{"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/media?parent=499"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/categories?post=499"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ingenieria.uner.edu.ar\/nucleos\/quimica_ambiental\/index.php\/wp-json\/wp\/v2\/tags?post=499"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}